Liver Metastases Potentiate Cancer Progression

Abstract Metastases are the primary cause of cancer mortality, and different sites metastasis associated with prognostic outcomes. In particular, liver metastases poor overall survival increased disease progression. It is known that suppress systemic antitumor immunity to limit immunotherapy efficacy on tumors through hepatic siphoning deletion CD8 +T cells, induction T regulatory depletion dendritic cells. However, it remains unclear how promote subsequent progression metastatic cascade. To investigate reprogram immune system potentiate progression, we developed a preclinical polymetastatic murine model lung metastases. Using B16F10 melanoma in syngeneic C57Bl/6 mice, observed presence burden. We hypothesized formation tumorigenic, immunosuppressive premetastatic niche distant sites. conducted single cell RNA (scRNA) sequencing flow cytometry profiling liver, peripheral blood, lungs mice or without Together, these data suggest an association between Ly6g +Ly6c −neutrophils Future efforts will focus defining contribution neutrophils ultimate goal understanding addressing immunosuppression promoted by